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Article Dans Une Revue Molecular Biology of the Cell Année : 2005

A small C-terminal sequence of Aurora B is responsible for localization and function.

Résumé

Aurora B, a protein kinase required in mitosis, localizes to inner centromeres at metaphase and the spindle midzone in anaphase and is required for proper chromosome segregation and cytokinesis. Aurora A, a paralogue of Aurora B, localizes instead to centrosomes and spindle microtubules. Except for distinct N termini, Aurora B and Aurora A have highly similar sequences. We have combined small interfering RNA (siRNA) ablation of Aurora B with overexpression of truncation mutants to investigate the role of Aurora B sequence in its function. Reintroduction of Aurora B during siRNA treatment restored its localization and function. This permitted a restoration of function test to determine the sequence requirements for Aurora B targeting and function. Using this rescue protocol, neither N-terminal truncation of Aurora B unique sequence nor substitution with Aurora A N-terminal sequence affected Aurora B localization or function. Truncation of unique Aurora B C-terminal sequence from terminal residue 344 to residue 333 was without effect, but truncation to 326 abolished localization and function. Deletion of residues 326-333 completely abolished localization and blocked cells at prometaphase, establishing this sequence as critical to Aurora B function. Our findings thus establish a small sequence as essential for the distinct localization and function of Aurora B.

Dates et versions

hal-00187757 , version 1 (15-11-2007)

Identifiants

Citer

Laetitia Scrittori, Dimitrios A Skoufias, Fabienne Hans, Véronique Gerson, Paolo Sassone-Corsi, et al.. A small C-terminal sequence of Aurora B is responsible for localization and function.. Molecular Biology of the Cell, 2005, 16 (1), pp.292-305. ⟨10.1091/mbc.E04-06-0447⟩. ⟨hal-00187757⟩
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