Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: a quantitative study. - Institut Albert Bonniot Accéder directement au contenu
Article Dans Une Revue Cytogenetics and Cell Genetics Année : 2001

Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: a quantitative study.

Résumé

Image restoration approaches, such as digital deconvolution, are becoming widely used for improving the quality of microscopic images. However, no quantification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25% smaller, and 1.2-kb fragment signals on combed molecules faithfully display the expected length.

Domaines

Imagerie
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Dates et versions

hal-00192532 , version 1 (28-11-2007)

Identifiants

Citer

K. Monier, L. Heliot, C. Rougeulle, E. Heard, M. Robert-Nicoud, et al.. Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: a quantitative study.. Cytogenetics and Cell Genetics, 2001, 92 (1-2), pp.59-62. ⟨10.1159/000056869⟩. ⟨hal-00192532⟩
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