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Article Dans Une Revue Plant, Cell and Environment Année : 2012

Nitric oxide production mediates oligogalacturonide-triggered immunity and resistance to Botrytis cinerea in Arabidopsis thaliana

Olivier Lamotte
Benoît Poinssot
Sylvain Jeandroz

Résumé

Nitric oxide (NO) regulates a wide range of plant processes from development to environmental adaptation. In this study, we investigated the production and/or function of NO in [i]Arabidopsis thaliana[/i] leaf discs and plants elicited by oligogalacturonides (OGs) and challenged with [i]Botrytis cinerea[/i]. We provided evidence that OGs triggered a fast and long lasting NO production which was Ca2+ dependent and involved nitrate reductase (NR). Accordingly, OGs triggered an increase of both NR activity and transcript accumulation. NO production was also sensitive to the mammalian NO synthase inhibitor L-NAME. Intriguingly, we showed that L-NAME affected NO production by interfering with NR activity, thus questioning the mechanisms of how this compound impairs NO synthesis in plants. We further demonstrated that NO modulates RBOHD-mediated reactive oxygen species (ROS) production and participates in the regulation of OG-responsive genes such as anionic peroxidase (PER4) and a beta-1,3-glucanase. Mutant plants impaired in PER4 and beta-1,3-glucanase, as well as Col-0 plants treated with the NO scavenger cPTIO, were more susceptible to [i]B. cinerea.[/i] Taken together, our investigation deciphers part of the mechanisms linking NO production, NO-induced effects and basal resistance to[i] B. cinerea[/i]
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Dates et versions

hal-01137174 , version 1 (30-03-2015)

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Sumaira Rasul, Carole Dubreuil, Olivier Lamotte, Emmanuel Koen, Benoît Poinssot, et al.. Nitric oxide production mediates oligogalacturonide-triggered immunity and resistance to Botrytis cinerea in Arabidopsis thaliana. Plant, Cell and Environment, 2012, 35 (8), pp.1483-1499. ⟨10.1111/j.1365-3040.2012.02505.x⟩. ⟨hal-01137174⟩
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