Erythrocyte phospholipid and polyunsaturated fatty acid composition in diabetic retinopathy - Université de Bourgogne Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2014

Erythrocyte phospholipid and polyunsaturated fatty acid composition in diabetic retinopathy

Résumé

Background: Long chain polyunsaturated fatty acids (LCPUFAs) including docosahexaenoic acid and arachidonic acid are suspected to play a key role in the pathogenesis of diabetes. LCPUFAs are known to be preferentially concentrated in specific phospholipids termed as plasmalogens. This study was aimed to highlight potential changes in the metabolism of phospholipids, and particularly plasmalogens, and LCPUFAs at various stages of diabetic retinopathy in humans. Methodology and Principal Findings: We performed lipidomic analyses on red blood cell membranes from controls and mainly type 2 diabetes mellitus patients with or without retinopathy. The fatty acid composition of erythrocytes was determined by gas chromatography and the phospholipid structure was determined by liquid chromatography equipped with an electrospray ionisation source and coupled with a tandem mass spectrometer (LC-ESI-MS/MS). A significant decrease in levels of docosahexaenoic acid and arachidonic acid in erythrocytes of diabetic patients with or without retinopathy was observed. The origin of this decrease was a loss of phosphatidyl-ethanolamine phospholipids esterified with these LCPUFAs. In diabetic patients without retinopathy, this change was balanced by an increase in the levels of several phosphatidyl-choline species. No influence of diabetes nor of diabetic retinopathy was observed on the concentrations of plasmalogen-type phospholipids. Conclusions and Significance: Diabetes and diabetic retinopathy were associated with a reduction of erythrocyte LCPUFAs in phosphatidyl-ethanolamines. The increase of the amounts of phosphatidyl-choline species in erythrocytes of diabetic patients without diabetic retinopathy might be a compensatory mechanism for the loss of LC-PUFA-rich phosphatidyl-ethanolamines.
Fichier principal
Vignette du fichier
koehrer_2014_vol9_e106912_plosone_{5C4B7F64-56E8-45A2-B24B-6E251B9C2DE1}.pdf (657.05 Ko) Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Loading...

Dates et versions

hal-01222439 , version 1 (29-10-2015)

Identifiants

Citer

Philippe Koehrer, Sara Saab, Olivier Berdeaux, Rodica Isaico, Stéphane Grégoire, et al.. Erythrocyte phospholipid and polyunsaturated fatty acid composition in diabetic retinopathy. PLoS ONE, 2014, 9 (9), pp.1-13. ⟨10.1371/journal.pone.0106912⟩. ⟨hal-01222439⟩
165 Consultations
71 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More