%0 Journal Article %T FT-IR spectroscopy : A powerful tool for studying the inter- and intraspecific biodiversity of cultivable non-Saccharomyces yeasts isolated from grape must %+ Vin Aliment Microbiologie et Stress (VAlMiS) %+ Institut für Mikrobiologie & Biochemie, Zentrum für Analytische Chemie und Mikrobiologie (IMB) %+ Agroécologie [Dijon] %A Grangeteau, Cédric %A Gerhards, Daniel %A Terrat, Sebastien %A Dequiedt, Samuel, S. %A Alexandre, Hervé %A Guilloux-Benatier, Michèle %A von Wallbrunn, Christian %A Rousseaux, Sandrine %Z Regional Council of Burgundy %Z Interprofessional Office of Burgundy wines %< avec comité de lecture %@ 0167-7012 %J Journal of Microbiological Methods %I Elsevier %V 121 %P 50 - 58 %8 2016-02 %D 2016 %R 10.1016/j.mimet.2015.12.009 %M 26688103 %K Transform-infrared-spectroscopy %K Pyrosequencing %K Strain typing %K Candida %K Green lacewings %K Intragenomic variation %K Sp-nov. %K Microbial ecology %K Candida-zemplinina %K Alcoholic fermentation %K FT-IR spectroscopy %K Wine yeast %K Identification %K Pyrolysis mass-spectrometry %K Botrytis-affected wine %Z Life Sciences [q-bio]/Food and Nutrition %Z Life Sciences [q-bio]/Microbiology and ParasitologyJournal articles %X The efficiency of the FT-IR technique for studying the inter- and intra biodiversity of cultivable non-Saccharomyces yeasts (NS) present in different must samples was examined. In first, the capacity of the technique FF-IR to study the global diversity of a given sample was compared to the pyrosequencing method, used as a reference technique. Seven different genera (Aureobasidium, Candida, Cryptococcus, Hanseniaspora, Issatchenkia, Metschnikowia and Pichia) were identified by FT-IR and also by pyrosequencing. Thirty-eight other genera were identified by pyrosequencing, but together they represented less than 6% of the average total population of 6 musts. Among the species identified, some of them present organoleptic potentials in winemaking, particularly Starmerella bacillaris (synonym Candida zemplinina). So in a second time, we evaluated the capacity of the FT-IR technique to discriminate the isolates of this species because few techniques were able to study intraspecific NS yeast biodiversity. The results obtained were validated by using a classic method as ITS sequencing. Biodiversity at strain level was high: 19 different strains were identified from 58 isolates. So, FT-IR spectroscopy seems to be an accurate and reliable method for identifying major genera present in the musts. The two biggest advantages of the FT-IR are the capacity to characterize intraspecific biodiversity of non-Saccharomyces yeasts and the possibility to discriminate a lot of strains. %G English %L hal-01446318 %U https://u-bourgogne.hal.science/hal-01446318 %~ UNIV-BOURGOGNE %~ INRA %~ PAM %~ AGREENIUM %~ AGROSUP-DIJON %~ INRAE %~ INSTITUT-AGRO-DIJON %~ INSTITUT-AGRO-DIJON-PAM %~ INSTITUT-AGRO-PAM %~ AGROECOLOGIE %~ INSTITUT-AGRO