Mitochondrial dysfunction, oxidative stress and apoptotic induction in microglial BV-2 cells treated with sodium arsenate. - Université de Bourgogne Accéder directement au contenu
Article Dans Une Revue Journal of Environmental Sciences Année : 2017

Mitochondrial dysfunction, oxidative stress and apoptotic induction in microglial BV-2 cells treated with sodium arsenate.

Résumé

The treatment of microglial BV-2 cells with sodium arsenate (As(V): 0.1-400μmol/L - 48hr) induces a dose-dependent response. The neurotoxic effects of high concentrations of As(V) (100, 200 and 400μmol/L) are characterized by increased levels of mitochondrial complexes I, II, and IV followed by increased superoxide anion generation. Moreover, As(V) triggers an apoptotic mode of cell death, demonstrated by an apoptotic SubG1 peak, associated with an alteration of plasma membrane integrity. There is also a decrease in transmembrane mitochondrial potential and mitochondrial adenosine triphosphate ATP. It is therefore tempting to speculate that As(V) triggers mitochondrial dysfunction, which may lead to defective oxidative phosphorylation subsequently causing mitochondrial oxidative damage, which in turn induces an apoptotic mode of cell death.
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Dates et versions

hal-01463001 , version 1 (09-02-2017)

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Wafa Kharroubi, Samia Haj Ahmed, Thomas Nury, Pierre Andreoletti, Rachid Sakly, et al.. Mitochondrial dysfunction, oxidative stress and apoptotic induction in microglial BV-2 cells treated with sodium arsenate.. Journal of Environmental Sciences, 2017, 51, pp.44-51. ⟨10.1016/j.jes.2016.08.028⟩. ⟨hal-01463001⟩
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