Structural and enzymatic characterization of a Drosophila melanogaster glutathione transferase overexpressed in the sensory organs after exposure to bitter molecules in food - Université de Bourgogne Accéder directement au contenu
Poster De Conférence Année : 2016

Structural and enzymatic characterization of a Drosophila melanogaster glutathione transferase overexpressed in the sensory organs after exposure to bitter molecules in food

Résumé

Glutathione transferases (GSTs) are ubiquitous key detoxification enzymes that catalyze the conjugation of glutathione to a large variety of xenobiotic chemical including odorants and sapid molecules. A previous study in Drosophila melanogaster demonstrated the existence of a GST specifically expressed in the antenna and involved in signal termination by catalyzing biotransformation of odorant molecules. Genomic analysis allows identifying forty GSTs in Drosophila melanogaster but their biochemistry is poorly documented. Here we demonstrated that among the GSTs, dmelGSTD2-2 was strongly and preferentially overexpressed in the sensory organs (antennae, proboscis, legs, wings) after an exposure to bitter molecules supplemented in food. In regard to these aspects we shed light on the existence of a gustatory GST. We expressed, purified and performed enzymatic characterization revealing that dmelGSTD2-2 is able to interact and biotransform bitter compounds (such as caffeine or isothiocyanates). Moreover, we solved the crystal structure of this dmelGST D2-2 which is the first of this enzyme class and also one of the first in insect. DmelGSTD2-2 was solved at 1.5 Å resolution and appears as a dimer. The biochemical function in Drosophila melanogaster of this enzyme toward bitter compounds is discussed in the light of the in vivo study supplemented by in silico binding analysis and enzymatic assay with a panel of bitter molecules.
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Dates et versions

hal-01579982 , version 1 (31-08-2017)

Identifiants

  • HAL Id : hal-01579982 , version 1
  • PRODINRA : 356933
  • WOS : 000386126000192

Citer

Daniel Gonzalez, Stéphane Fraichard, Jean-François Ferveur, Yves Artur, Loïc Briand, et al.. Structural and enzymatic characterization of a Drosophila melanogaster glutathione transferase overexpressed in the sensory organs after exposure to bitter molecules in food. 17. international symposium on Olfaction and taste (ISOT), Jun 2016, Yokohama, Japan. Oxford University Press, Chemical Senses, 41 (9), 1 p., 2016, Chemical Senses. ⟨hal-01579982⟩
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