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Article Dans Une Revue Water Research Année : 2023

Effect of a karst system (France) on extended spectrum beta-lactamase (ESBL)-producing Escherichia coli

Résumé

Karst aquifers are an important water resource worldwide particularly exposed to anthropogenic pollution, including antibiotic-resistance. The release of antibiotic-resistant bacterial pathogens in the environment is a major public health challenge worldwide. In this One Health study, we aimed to determine the effect of karst on antibiotic-resistant bacteria. For this purpose, we determined the concentrations of extended-spectrum β-lactamases-producing Escherichia coli (ESBL-Ec) for 92 weeks in a rural karst hydrosystem providing drinking water. ESBL-Ec isolates (n = 130) were sequenced by whole genome sequencing. We analysed the isolates at different levels of granularity, i.e., phylogroup, sequence type, presence of antibiotic-resistance genes, mutations conferring antibiotic-resistance, and virulence genes. The ESBL-Ec concentrations were spatially and temporally heterogeneous in the studied karst hydrosystem. ESBL-Ec isolates survived in the karst and their concentrations were mostly explained by the hydrodynamic of the hydrosystem. We demonstrate that the studied karst has no filtration effect on ESBL-Ec, either quantitatively (i.e., in the ESBL-Ec concentrations) or qualitatively (i.e., in the genetic characteristics of ESBL-Ec isolates).
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Dates et versions

hal-04157633 , version 1 (10-07-2023)

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Charles Henriot, Hélène Celle, Victor Klaba, Adrien Biguenet, Cécile Miège, et al.. Effect of a karst system (France) on extended spectrum beta-lactamase (ESBL)-producing Escherichia coli. Water Research, 2023, 230, pp.119582. ⟨10.1016/j.watres.2023.119582⟩. ⟨hal-04157633⟩
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