Identification and functional characterization of S-nitrosated proteins from Klebsormidium nitens - Université de Bourgogne Accéder directement au contenu
Communication Dans Un Congrès Année : 2022

Identification and functional characterization of S-nitrosated proteins from Klebsormidium nitens

Zoé Chaudron
  • Fonction : Auteur
  • PersonId : 1198950
Valérie Nicolas-Francès
  • Fonction : Auteur
  • PersonId : 1129953
Agnès Klinguer
  • Fonction : Auteur
  • PersonId : 1193956
Claire Rosnoblet
  • Fonction : Auteur
  • PersonId : 1206638
Angélique Besson-Bard
  • Fonction : Auteur
  • PersonId : 1202782
David Wendehenne
  • Fonction : Auteur
  • PersonId : 1198880

Résumé

The small gaseous molecule nitric oxide (NO) is well established as a major ubiquitous component of cell signalling. A key signalling mechanism mediating NO effects is S-nitrosation, a post-translational modification by which NO can impact the target protein activities, subcellular localizations and capacities to form protein complexes. The identification of proteins targeted by NO is of major interest in order to elucidate NO functions. Interestingly, land plants lack NO synthase (NOS), which is the main enzyme for NO synthesis in metazoans, while a few algal species possess it, thus raising many interrogations. Therefore, we focused on the identification of S-nitrosated proteins during salt stress in Klebsormidium nitens, a freshwater algal species possessing a NOS and established as a model to study plants adaptation to land. We applied the Biotin Switch method followed by mass spectrometry analysis. This method allows the purification of S-nitrosated proteins. We found 43 proteins with significantly higher S-nitrosation levels in salt response condition. Orthology analysis were performed against the model plant Arabidopsis thaliana, in order to determine the potential function of these proteins. Among them, three were selected for being potentially involved in cell signaling. We aim to produce them in bacteria, in order to measure their activity and observe the impact of the S-nitrosation on it.
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Dates et versions

hal-03982477 , version 1 (10-02-2023)

Identifiants

  • HAL Id : hal-03982477 , version 1

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Zoé Chaudron, Valérie Nicolas-Francès, Agnès Klinguer, Claire Rosnoblet, Angélique Besson-Bard, et al.. Identification and functional characterization of S-nitrosated proteins from Klebsormidium nitens. Journée des Doctorants UMR Agroécologie 2022, Nov 2022, Dijon, France. ⟨hal-03982477⟩
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