Improvement of Treg immune response after treatment with tocilizumab in giant cell arteritis - Université de Bourgogne Accéder directement au contenu
Article Dans Une Revue Clinical & Translational Immunology Année : 2021

Improvement of Treg immune response after treatment with tocilizumab in giant cell arteritis

Kim Heang Ly
  • Fonction : Auteur
Eric Liozon
  • Fonction : Auteur
Benjamin Terrier

Résumé

OBJECTIVES: To study the percentage, suppressive function and plasticity of Treg in giant cell arteritis (GCA), and the effects of glucocorticoids and tocilizumab. METHODS: Blood samples were obtained from 40 controls and 43 GCA patients at baseline and after treatment with glucocorticoids + IV tocilizumab (n = 20) or glucocorticoids (n = 23). Treg percentage and phenotype were assessed by flow cytometry. Suppressive function of Treg was assessed by measuring their ability to inhibit effector T-cell (Teff) proliferation and polarisation into Th1 and Th17 cells. RESULTS: Treg (CD4(+)CD25(high)FoxP3(+)) frequency in total CD4(+) T cells was decreased in active GCA patients when compared to controls (2.5% vs. 4.7%, P < 0.001) and increased after treatment with tocilizumab but worsened after treatment with glucocorticoids alone. Treg lacking exon 2 of FoxP3 were increased in GCA patients when compared to controls (23% vs. 10% of total Treg, P = 0.0096) and normalised after treatment with tocilizumab + glucocorticoids but not glucocorticoids alone. In GCA patients, Treg were unable to control Teff proliferation and induced ˜50% increase in the amount of IL-17(+) Teff, which was improved after in vitro blockade of the IL-6 pathway by tocilizumab. CONCLUSION: This study reports quantitative and functional disruptions in the regulatory immune response of GCA patients and demonstrates that, unlike glucocorticoids, tocilizumab improves Treg immune response.

Domaines

Immunologie

Dates et versions

hal-03356648 , version 1 (28-09-2021)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Maxime Samson, Hélène Greigert, Marion Ciudad, Claire Gerard, Thibault Ghesquière, et al.. Improvement of Treg immune response after treatment with tocilizumab in giant cell arteritis. Clinical & Translational Immunology, 2021, 10 (9), pp.e1332. ⟨10.1002/cti2.1332⟩. ⟨hal-03356648⟩
16 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More